Products & Application

Silica Gel Powder For TLC

Thin-layer chromatography is performed on a sheet or plate (about 0.25mm thick) of glass, aluminum foil or plastic, coated with a thin layer of solid ADSORBENT material. The adsorbent material is usually silica gel 400-800 mesh size with a binder or without a binder, silica gel with a binder is known as Silica Gel G TLC grade as it contains Gypsum as a binder, which will allow the silica to adhere to the plates, for non-colored compound fluorescence is added for easy analysis of the compound under the ultraviolet chamber, silica-containing binder and fluorescence is known as silica gel GF254. The silica gel without binder is known is Silica Gel H TLC Grade. The silica gel H grade containing fluorescence activity is known as Silica Gel HF254.

TLC method is developed to see the progress in the reactions between compound to the adsorbent, mixture, and solvents. These reactions help to identify the elements present in a mixture of compounds with the percentage of purity. TLC is a part of organic chemicals used in the separation, identification of different compounds.

This grade of Silica Gel is suitable for making TLC plates. It allows you to prepare your own customized TLC plates, either on Glass, Plastic or Aluminium sheets, with your desired thickness. A binder Gypsum (G) is added to this Silica Gel grade, which helps the Silica Gel to firmly settle on the TLC sheet. This grade also assures a better Rf value for the compounds which get separated. It is easy to distinguish between very closely related compounds.

Parameters Silica Gel Chromatography Grades
Grades Silica Gel H Silica Gel G Silica Gel GF254 Silica Gel HF254
Assay (as SIO2 ) 98-99% 98-99% 98-99% 98-99%
Ph 7+-0.5 7+-0.5 7+-0.5 7+-0.5
Bulk Density (g/ml) 0.45-0.60 0.45-0.60 0.45-0.60 0.45-0.60
Loss of Drying % <6 <6 <6 <6
Adsorption Capacity at 100% Humidity 30-35 30-35 30-35 30-35
Friability 99.95 99.95 99.95 99.95
Chloride (NaCl) 0.02% 0.02% 0.02% 0.02%
Sulfates (Na2 SO4) 0.02% 0.02% 0.02% 0.02%
Binder as CaSo4 NIL 13%+1 13%+1 NIL
Particle Size 300-400 -350 -350 -350
Silica Gel Powder For TLC

TLC Plates

Thin-layer chromatography, or TLC, is a methodology for analyzing mixtures by separating the compounds within the mixture. TLC can be used to help determine the number of components in a mixture, verify elements of compounds, and also the purity of a compound.

One of the foremost necessary applications of TLC is in the separation of multicomponent pharmaceutical formulations. In the food and cosmetic industry, TLC method is used for separation and identification of colors, preservatives, sweetening agent, and numerous cosmetic product.