Silica Gel Mesh Size 60-120 For Chromatography
Silica gel or silicon dioxide gel has found its application in numerous different types of chromatography techniques due to its high absorbency properties, highly porous structure, and non-reactive nature. It is a flexible and multifunctional stationary phase adsorbent material that is suitable for the separation of a range of different compounds, organic molecules, inorganic chemical compositions, and biological molecules efficiently from a particular mixture or chemical fusion/blend, due to its high affinity properties.
Silica gel is available in various pore sizes and these sizes can be used in various different chromatography applications, unique to its application and use for the separation, clarification, and purification of various chemical compounds. Though, Silica gel comes in a wide range of mesh sizes, the use of silica gel mesh size 60-120 for chromatography techniques is quite common in many applications, especially for the separation of compounds from a specific mixture.
Understanding Silica Gel Mesh Size 60-120
The mesh size of a silica gel adsorbent material, that is vividly used as a stationary phase in chromatography, is a measure of the particle size distribution throughout the material. This mesh size is usually expressed in terms of a range, ranging from 60 to 120 mesh, with 60 mesh representing the lower limit of finer particles and 120 mesh representing the upper limit of coarse particles.
Silica gel mesh size 60-120 has many useful features, that are efficient in the chromatography process, to help separate compounds using the stationary and mobile phase of the process. Silica gel used in the stationary phase acts as a great adsorbent phase where the mixture is passed through the column and can separate different compounds depending on their affinity, size, polarity, charge, contamination levels, and compositions.
This mesh size of silica gel has a range, and thus can efficiently separate particles of different sizes, and provides high resolution for chromatographic separation of those fine particulates from a specific mixture. This allows for the effective separation of closely related compounds within a complex, that may be otherwise difficult to separate, making silica gel a preferred material for analytical chromatography of a particular mixture.
During the chromatography process, using the bigger particle size means you can load more samples without having to worry about too much pressure dropping inside the column during the chromatography process, which is great for preparative chromatography which generally deals with bigger sample sizes.
This mesh size of 60-120 in silica gel is perfect for both analytical and preparation chromatography applications, as it has a good flow rate and high separation efficiency, making it ideal for the varying applications used in chromatography techniques.
60-120 Silica gel can work with and is compatible with both polar and non-polar solvents. It is compatible with a broad range of sample types including small molecules, bigger compounds, and complex mixtures.
Uses and Applications of Silica Gel Mesh Size 60-120 in Column Chromatography
Silica gel 60-120 is used in many chromatography techniques such as Flash chromatography, Column chromatography, Thin-layer chromatography, gravity column chromatography, as well as other industrial applications, preparative and analytical chromatography. In column chromatography, silica gel 60-120 mesh size is widely used. It is a highly sought-after option due to its ability to provide a balanced resolution with flow rate. Its small particle size allows for a high degree of resolution, yet does not lead to excessive backpressure.
Silica gel mesh sizes ranging from 60 to 120 are suitable for the purification of a broad range of organic compounds, ranging from pharmaceuticals to natural products and synthetic compounds.
Silica gel mesh sizes ranging from 60-120 microns is widely used and applied in various chemical laboratories to help in the purification process of a variety of applications, including the isolation of different natural products, the synthesis of specialized chemicals, and the purification of reaction mixtures through the chromatography process.
How to Use Silica Gel Mesh Size 60-120 for Column Chromatography?
Select a suitable column with the desired dimensions, according to its length, diameter, etc. You can add a small amount of glass wool at the bottom of the column to prevent it from spilling out, and then fill the column with 60 to 120 mesh sizes of silica gel adsorbent material to create a uniform, tightly packed column for the efficient functioning of the column chromatography process.
You can now load the sample from the top of the column, and choose and dissolve the sample in a suitable solvent, and make sure the gel bed is not disturbed or damaged while loading the column.
Now, allow the solvent to flow through the column, taking the sample with through the adsorbent material in the column. You can adjust the flow rate by either using gravity or a pump, depending on the column size and purpose of the chromatography technique. You can monitor the elution process, collecting fractions as different compounds exit the column.
You can collect fractions in test tubes/containers as distinct and separated compounds eluted from the column towards the end of the chromatography process, and then label each fraction according to the elution order and time of collection of these separated compounds from the mixture.
Analyze the collected fractions using appropriate analytical techniques, such as thin-layer chromatography or high-performance liquid chromatography, to identify the compounds and assess their purity.
If you're looking for a silica gel mesh size that can be used for column chromatography efficiently, then you should definitely consider Silica gel mesh sizes 60-120 as it is super versatile and powerful in nature. The material is great for separating, purifying, and analyzing many different kinds of compounds.