Silica Gel TLC Plates
Thin-layer chromatography is performed on a sheet or plate (about 0.25mm thick) of glass, aluminium foil or plastic coated with a thin layer of solid ADSORBENT material. The adsorbent material is usually silica gel 400-800 mesh size with or without a binder. Silica gel with a binder is known as Silica Gel G TLC grade as it contains Gypsum as a binder, which will allow the silica to adhere to the plates, for non-coloured compound fluorescence is added for easy analysis of the compound under the ultraviolet chamber, silica-containing binder and fluorescence is known as silica gel GF254. The silica gel without binder is known is Silica Gel H TLC Grade. The silica gel H grade containing fluorescence activity is known as Silica Gel HF254.
The TLC method is developed to see the progress in the reactions between compounds to the adsorbent, mixture, and solvents. These reactions help to identify the elements present in a mixture of compounds with a purity percentage. TLC is a part of organic chemicals that separate and identify different compounds.
This grade of Silica Gel is suitable for making TLC plates. It allows you to prepare your own customized TLC plates, on Glass, Plastic or Aluminium sheets, with your desired thickness. A binder Gypsum (G) is added to this Silica Gel grade, which helps the Silica Gel to settle on the TLC sheet firmly. This grade also assures a better Rf value for the separated compounds. It is easy to distinguish between very closely related compounds.
|Parameters||Silica Gel Chromatography Grades|
|Grades||Silica Gel H||Silica Gel G||Silica Gel GF254||Silica Gel HF254|
|Assay (as SIO2 )||98-99%||98-99%||98-99%||98-99%|
|Bulk Density (g/ml)||0.45-0.60||0.45-0.60||0.45-0.60||0.45-0.60|
|Loss of Drying %||<6||<6||<6||<6|
|Adsorption Capacity at 100% Humidity||30-35||30-35||30-35||30-35|
|Sulfates (Na2 SO4)||0.02%||0.02%||0.02%||0.02%|
|Binder as CaSo4||NIL||13%+1||13%+1||NIL|
Silica Gel Powder For TLC
Thin-layer chromatography, or TLC, is a methodology for analysing mixtures by separating the compounds within the mixture. TLC helps to determine the number of components in a mixture and verify elements of compounds and the purity of a compound.
One of the foremost necessary applications of TLC is to separate the multicomponent pharmaceutical formulations. This method is used in the food and cosmetic industry for separating and identifying colours, preservatives, sweetening agents, and numerous cosmetic products.